Article
Formation of the Ras dimer is essential for Raf-1 activation.
The Journal of biological chemistry - 11 Feb 2000
Inouye K, Mizutani S, Koide H, Kaziro Y
Abstract excerpt
Although it is well established that Ras requires membrane localization for activation of its target molecule, Raf-1, the reason for this requirement is not fully understood. In this study, we found that modified Ras, which is purified from Sf9 cells, could activate Raf-1 in a cell-free system, when incorporated into liposome. Using a bifunctional cross-linker and a protein-fragmentation complementation assay, we...
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