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Article

Structural determinants of Ras-Raf interaction analyzed in live cells.

Molecular biology of the cell - 1 Jul 2002

Bondeva Tzvetanka, Balla András, Várnai Péter, Balla Tamas

Abstract excerpt

The minimum structure of the Raf-1 serine/threonine kinase that recognizes active Ras was used to create a green fluorescent fusion protein (GFP) for monitoring Ras activation in live cells. In spite of its ability to bind activated Ras in vitro, the Ras binding domain (RBD) of Raf-1 (Raf-1[51-131]GFP) failed to detect Ras in Ras-transformed NIH 3T3 fibroblasts and required the addition of the cysteine-rich...

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Identifiers

PMID: 12134072
DOI: 10.1091/mbc.e02-01-0019
PMCID: PMC117316

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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