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Article

Investigation of a genetically engineered mutant of barnacle troponin C containing a central helix deletion.

Pflugers Archiv : European journal of physiology - 1 Dec 1999

Allhouse L D, Miller T, Li Q, Guzman G, Potter J D, Mandveno A, Ashley C C

Abstract excerpt

To examine the importance of the central alpha-helix of troponin C (TnC) we have bacterially expressed one of the isoforms of barnacle TnC (BTnC2), BTnCWT, but without the aspartate residue at position 80 in the central helix (BTnC80-). This manipulation is expected to produce an approximately 100 degrees axial rotation of the C-domain with respect to the N-domain, and a net charge change of -1. BTnC80- mutant...

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Identifiers

PMID: 10651002
DOI: 10.1007/s004249900133

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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