Article
Characterisation of a mutant of barnacle troponin C lacking Ca2+-binding sites at positions II and IV.
Pflugers Archiv : European journal of physiology - 1 Jun 1999
Allhouse L D, Guzman G, Miller T, Li Q, Potter J D, Ashley C C
Abstract excerpt
This study investigates a mutant barnacle troponin C (TnC) protein (BTnC2-4-) in which the Ca2+-binding sites (sites II and IV) have been rendered non-functional. Eliminating Ca2+ binding at both Ca2+-binding sites of barnacle TnC did not prevent the incorporation of BTnC2-4- into TnC-depleted myofibrillar bundles, although, as expected, the mutant was not able to effect muscle regulation. We conclude that the...
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