Article
Ca2(+)-dependent interactions between the C-helix of troponin-C and troponin-I. Photocross-linking and fluorescence studies using a recombinant troponin-C.
The Journal of biological chemistry - 25 Mar 1990
Wang Z Y, Sarkar S, Gergely J, Tao T
Abstract excerpt
We have used in vitro mutagenesis to synthesize in Escherichia coli a recombinant rabbit skeletal troponin-C (designated as TnC57) in which Cys-98 was replaced with leucine, and Ala-57 in the C-helix of the N-terminal domain was replaced with cysteine. TnC57 labeled with the bifunctional photocro...
Topics
- Animals
- Benzophenones
- Calcium
- Cross-Linking Reagents
- Escherichia coli
- Kinetics
- Macromolecular Substances
- Maleimides
- Muscles
- Mutation
- Photochemistry
- Rabbits
- Recombinant Proteins
- Spectrometry, Fluorescence
- Troponin
- Troponin C
- Troponin I