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Article

The efficiency of Escherichia coli selenocysteine insertion is influenced by the immediate downstream nucleotide.

Nucleic acids research - 1 Feb 2000

Sandman K E, Noren C J

Abstract excerpt

Selenocysteine (Sec) incorporation requires the TGA opal codon and a downstream Sec insertion sequence (SECIS), which can be partially randomized and cloned into M13 pIII fusion constructs for phage display. This combinatorial approach provides a convenient non-radioactive assay that couples phage production to opal suppression. Two SECIS libraries were prepared, with the immediate downstream nucleotide either...

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Identifiers

PMID: 10637327
DOI: 10.1093/nar/28.3.755
PMCID: PMC102542

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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