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Article

Revised Escherichia coli selenocysteine insertion requirements determined by in vivo screening of combinatorial libraries of SECIS variants.

Nucleic acids research - 15 Apr 2003

Sandman Karen E, Tardiff Daniel F, Neely Lori A, Noren Christopher J

Abstract excerpt

To investigate the stringency of the Escherichia coli selenocysteine insertion sequence (SECIS) requirements, libraries of SECIS variants were screened via a novel method in which suppression of the selenocysteine (Sec) opal codon was coupled to bacteriophage plaque formation. The SECIS variant libraries were designed with a mostly paired lower stem, so that randomization could be focused on the upper stem and...

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Identifiers

PMID: 12682374
DOI: 10.1093/nar/gkg304
PMCID: PMC153732

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Source receipt: pubmed, retrieved 7 Sept 2026.

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