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Article

Functional and nonfunctional mutations distinguished by random recombination of homologous genes.

Proceedings of the National Academy of Sciences of the United States of America - 22 Jul 1997

Zhao H, Arnold F H

Abstract excerpt

We describe a convenient method for distinguishing functional from nonfunctional or deleterious mutations in homologous genes. High fidelity in vitro gene recombination ("DNA shuffling") coupled with sequence analysis of a small sampling of the shuffled library exhibiting the evolved behavior allows identification of those mutations responsible for the behavior in a background of neutral and deleterious...

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Identifiers

PMID: 9223302
DOI: 10.1073/pnas.94.15.7997
PMCID: PMC21544

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Source receipt: pubmed, retrieved 7 Sept 2026.

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