Article
Functional and nonfunctional mutations distinguished by random recombination of homologous genes.
Proceedings of the National Academy of Sciences of the United States of America - 22 Jul 1997
Zhao H, Arnold F H
Abstract excerpt
We describe a convenient method for distinguishing functional from nonfunctional or deleterious mutations in homologous genes. High fidelity in vitro gene recombination ("DNA shuffling") coupled with sequence analysis of a small sampling of the shuffled library exhibiting the evolved behavior all...
Topics
- Calorimetry, Differential Scanning
- Enzyme Stability
- Mutagenesis, Site-Directed
- Mutation
- Recombination, Genetic
- Subtilisins