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Article

Evolutionary molecular engineering by random elongation mutagenesis.

Nature biotechnology - 1 Jan 1999

Matsuura T, Miyai K, Trakulnaleamsai S, Yomo T, Shima Y, Miki S, Yamamoto K, Urabe I

Abstract excerpt

We describe a new method of random mutagenesis that employs the addition of peptide tails with random sequences to the C-terminal of enzyme molecules. A mutant population of catalase I from Bacillus stearothermophilus prepared by this method has a diversity in thermostability and enzyme activity equal to that obtained after random point mutagenesis. When a triple mutant of catalase I (I108T/D130N/1222T)-the...

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PMID: 9920270
DOI: 10.1038/5232

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