Article
Improvement in the alkaline stability of subtilisin using an efficient random mutagenesis and screening procedure.
Protein engineering - 1 Jan 2000
Cunningham B C, Wells J A
Abstract excerpt
An efficient random mutagenesis procedure coupled to a replica plate screen facilitated the isolation of mutant subtilisins from Bacillus amyloliquefaciens that had altered autolytic stability under alkaline conditions. Out of about 4000 clones screened, approximately 70 produced subtilisins with reduced stability (negatives). Two clones produced a more stable subtilisin (positives) and were identified as having...
Topics
- Bacillus
- Enzyme Stability
- Mutagenicity Tests
- Mutation
- Subtilisins