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Article

Isolation and transformation of uracil auxotrophs of the edible basidiomycete Pleurotus ostreatus.

FEMS microbiology letters - 15 Dec 1999

Kim B G, Magae Y, Yoo Y B, Kwon S T

Abstract excerpt

Uracil auxotrophs of Pleurotus ostreatus were isolated using the selectable marker, resistance to 5'-fluoro-orotic acid (5'-FOA). Two of the nine uracil auxotrophs obtained were transformed to prototrophy using plasmid pTRura 3-2 that contains the orotidine monophosphate decarboxylase (ura3) gene from Trichoderma reesei. Southern blot analyses of the transformants showed that the transforming DNA had integrated...

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PMID: 10585542
DOI: 10.1111/j.1574-6968.1999.tb08848.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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