Article
Development of an improved variant of GH51 α-l-arabinofuranosidase from Pleurotus ostreatus by directed evolution.
New biotechnology - 25 May 2014
Giacobbe Simona, Vincent Florence, Faraco Vincenza
Abstract excerpt
In this study, the α-l-arabinofuranosidase from Pleurotus ostreatus was subjected to directed evolution by expressing a library of around 7000 randomly mutated variants by error prone Polymerase Chain Reaction. High-throughput screening of the library for the most active variants was performed by...
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