Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Improved electroporation-mediated non-integrative transformation of Thermomyces lanuginosus.

Journal of microbiological methods - 1 May 2009

Gangavaram Lakshmi Prasanna, Mchunu Nokuthula, Ramakrishnan Priya, Singh Suren, Permaul Kugen

Abstract excerpt

The development of an efficient fungal expression system for recombinant proteins requires an improved transformation system for the host organism. We report a facile, efficient and highly reproducible electroporation-mediated transformation system for Thermomyces lanuginosus with a transformation efficiency of 1.27 x 10(3) transformants/microg DNA. Conidia of T. lanuginosus were stably transformed to hygromycin...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Ascomycota
Share to Blotting, Southern

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Drug Resistance, Fungal

Share to Electroporation

Share to Genes, Fungal

Share to Phosphotransferases (Alcohol Group Acceptor)

Share to Reproducibility of Results

Share to Transformation, Genetic

Source

Identifiers

PMID: 19318045
DOI: 10.1016/j.mimet.2009.01.025

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Improved electroporation-mediated non-integrative transformation of Thermomyces lanuginosus.PMID 19318045Read ↗

Agrobacterium tumefaciens-mediated transformation of the lichen fungus, Umbilicaria muehlenbergii.Semantic proximity Read ↗

Constitutive alpha-amylase producing mutant and recombinant haploid strains of thermophilic fungus Thermomyces lanuginosus.Semantic proximity Read ↗

Development of a telomere vector-based approach to overcome limitations caused by lethal phenotypes in the study of essential genes in Magnaporthe oryzae.Semantic proximity Read ↗

A transformation system for the biocontrol yeast, Candida oleophila, based on hygromycin B resistance.Semantic proximity Read ↗

Methodologies to increase the transformation efficiencies and the range of bacteria that can be transformed.Semantic proximity Read ↗

A comparison of the phenotypic and genetic stability of recombinant Trichoderma spp. generated by protoplast- and Agrobacterium-mediated transformation.Semantic proximity Read ↗

Application of the red fluorescent protein mCherry in mycelial labeling and organelle tracing in the dermatophyte Trichophyton mentagrophytes.Semantic proximity Read ↗

Development of a sufficient and effective procedure for transformation of an oleaginous yeast, Rhodosporidium toruloides DMKU3-TK16.Semantic proximity Read ↗

Cloning and evaluation of different constitutive promoters in the oleaginous yeast Rhodosporidium toruloides.Semantic proximity Read ↗

Stable genetic transformation of garlic plants using particle bombardment.Semantic proximity Read ↗

Establishment of a new and efficient Agrobacterium-mediated transformation system in the nematicidal fungus Purpureocillium lilacinum.Semantic proximity Read ↗

Transformation and strain engineering of Tetrahymena.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.