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Article

Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes.

Current genetics - 1 May 1991

Bergès T, Barreau C

Abstract excerpt

Uridine auxotrophs of the filamentous fungus Trichoderma reesei have been selected using a positive screening procedure with 5-fluoro orotate. Mutants deficient for the orotidine-5'-phosphate decarboxylase gene (ura3 mutants) and for the orotate phosphoribosyl transferase gene (ura5 mutants) have been characterized. The homologous ura3 and ura5 genes have been isolated and used to transform the auxotrophic...

Topics

Amino Acid Sequence
Blotting, Southern
Cloning, Molecular
DNA, Fungal
Molecular Sequence Data
Mutation
Orotate Phosphoribosyltransferase
Orotidine-5'-Phosphate Decarboxylase
Plasmids
Restriction Mapping
Sequence Homology, Nucleic Acid

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Transformation, Genetic

Trichoderma

Uridine

Source

Identifiers

PMID: 1913875
DOI: 10.1007/BF00309596

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1913875-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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