The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on a pyrG-negative mutant strain.

doi:10.1007/BF00321118

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Article

The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on a pyrG-negative mutant strain.

Current genetics - 1 Jul 1990

Gruber F, Visser J, Kubicek C P, de Graaff L H

Abstract excerpt

Six uridine auxotroph mutants of Trichoderma reesei QM 9414 were isolated by resistance to 5-fluoroorotic acid and one strain was identified as OMP-decarboxylase negative (pyr-) by a radiometric enzyme assay. Transformation to uridine prototrophy was achieved with the pyr4 gene of Neurospora cras...

Topics

Blotting, Southern
Chromatography, Thin Layer
Drug Resistance, Microbial
Genes, Fungal
Mutation
Orotic Acid
Orotidine-5'-Phosphate Decarboxylase
Transformation, Genetic
Trichoderma
Uridine

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