Article
Homologous transformation of the edible basidiomycete Agrocybe aegerita with the URA1 gene: characterization of integrative events and of rearranged free plasmids in transformants.
Current genetics - 1 May 1994
Noël T, Labarère J
Abstract excerpt
The URA1 gene, encoding dihydroorotate dehydrogenase of the pyrimidine pathway, cloned into pUC18 (pUra1-1) was used to develop an homologous transformation system for the cultivated mushroom Agrocybe aegerita. Protoplasts of a ura1 auxotrophic strain were transformed by electroporation with efficiencies ranging from 1 to 26 transformants per micrograms of DNA. The phenotype of the stable Ura+ transformants...
Topics
- Agaricales
- DNA, Fungal
- Dihydroorotate Dehydrogenase
- Electroporation
- Escherichia coli
- Extrachromosomal Inheritance
- Genes, Fungal
- Genetic Vectors
- Mitosis
- Mutation
- Oxidoreductases