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Article

Transformation by complementation of a uracil auxotroph of the hyper lignin-degrading basidiomycete Phanerochaete sordida YK-624.

Applied microbiology and biotechnology - 1 Oct 2007

Yamagishi Kenji, Kimura Toshiyuki, Oita Sigeru, Sugiura Tatsuki, Hirai Hirofumi

Abstract excerpt

Phanerochaete sordida YK-624 is a hyper lignin-degrading basidiomycete possessing greater ligninolytic selectivity than either P. chrysosporium or Trametes versicolor. To construct a gene transformation system for P. sordida YK-624, uracil auxotrophic mutants were generated using a combination of ultraviolet (UV) radiation and 5-fluoroorotate resistance as a selection scheme. An uracil auxotrophic strain (UV-64)...

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PMID: 17701036
DOI: 10.1007/s00253-007-1093-7

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