Isolation and transformation of uracil auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium.

doi:10.1007/BF00310898

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Article

Isolation and transformation of uracil auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium.

Current genetics - 1 Jan 1993

Akileswaran L, Alic M, Clark E K, Hornick J L, Gold M H

Abstract excerpt

Uracil auxotrophs of Phanerochaete chrysosporium were isolated using 5-fluoroorotate resistance as a selection scheme. The ura3 auxotrophs deficient in orotidylate decarboxylase and ura5 auxotrophs deficient in orotate phosphoribosyl transferase were characterized by enzyme assays and complementa...

Topics

Ascomycota
Basidiomycota
Drug Resistance, Microbial
Genes, Fungal
Lignin
Mutation
Orotate Phosphoribosyltransferase
Orotic Acid
Orotidine-5'-Phosphate Decarboxylase
Recombination, Genetic
Transformation, Genetic
Uracil

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