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Article

In vivo screening of haloalkane dehalogenase mutants.

Bioorganic & medicinal chemistry - 1 Oct 1999

Chang C H, Schindler J F, Unkefer C J, Vanderberg L A, Brainard J R, Terwilliger T C

Abstract excerpt

Haloalkane dehalogenase (Dh1A) from Xanthobacter autotrophicus GJ10 catalyzes the dehalogenation of short chain primary alkyl halides. Due to the high Km and low turnover, wild type Dh1A is not optimal for applications in bioremediation. We have developed an in vivo screen, based on a colorimetric pH indicator, to identify Dh1A mutant with improved catalytic activity. After screening 50,000 colonies, we...

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Identifiers

PMID: 10579523
DOI: 10.1016/s0968-0896(99)00148-0

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