Article
Characterization of the haloacid dehalogenase from Xanthobacter autotrophicus GJ10 and sequencing of the dhlB gene.
Journal of bacteriology - 1 Dec 1991
van der Ploeg J, van Hall G, Janssen D B
Abstract excerpt
The haloacid dehalogenase of the 1,2-dichloroethane-utilizing bacterium Xanthobacter autotrophicus GJ10 was purified from a mutant with an eightfold increase in expression of the enzyme. The mutant was obtained by selecting for enhanced resistance to monobromoacetate. The enzyme was purified thro...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Genes, Bacterial
- Gram-Negative Aerobic Bacteria
- Hydrogen-Ion Concentration
- Hydrolases
- Molecular Sequence Data
- Mutation