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Article

Modification of activity and specificity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26 by engineering of its entrance tunnel.

The Journal of biological chemistry - 26 Dec 2003

Chaloupková Radka, Sýkorová Jana, Prokop Zbynek, Jesenská Andrea, Monincová Marta, Pavlová Martina, Tsuda Masataka, Nagata Yuji, Damborský Jirí

Abstract excerpt

Structural comparison of three different haloalkane dehalogenases suggested that substrate specificity of these bacterial enzymes could be significantly influenced by the size and shape of their entrance tunnels. The surface residue leucine 177 positioned at the tunnel opening of the haloalkane dehalogenase from Sphingomonas paucimobilis UT26 was selected for modification based on structural and phylogenetic...

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PMID: 14525993
DOI: 10.1074/jbc.M306762200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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