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Article

Adaptation of Xanthobacter autotrophicus GJ10 to bromoacetate due to activation and mobilization of the haloacetate dehalogenase gene by insertion element IS1247.

Journal of bacteriology - 1 Mar 1995

van der Ploeg J, Willemsen M, van Hall G, Janssen D B

Abstract excerpt

Monobromoacetate (MBA) is toxic for the 1,2-dichloroethane-degrading bacterium Xanthobacter autotrophicus GJ10 at concentrations higher than 5 mM. Mutants which are able to grow on higher concentrations of MBA were isolated and found to overexpress haloacid dehalogenase, which is encoded by the dhlB gene. In mutant GJ10M50, a DNA fragment (designated IS1247) had copied itself from a position on the chromosome...

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Identifiers

PMID: 7868610
DOI: 10.1128/jb.177.5.1348-1356.1995
PMCID: PMC176742

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