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Article

Generating segmental mutations in haloalkane dehalogenase: a novel part in the directed evolution toolbox.

Nucleic acids research - 15 Apr 2002

Pikkemaat Mariël G, Janssen Dick B

Abstract excerpt

Directed evolution techniques allow us to genuinely mimic molecular evolution in vitro. To enhance this imitation of natural evolutionary processes on a laboratory scale in even more detail, we developed an in vitro method for the generation of random deletions and repeats. The pairwise fusion of two fragments of the same gene that are truncated by exonuclease BAL-31 either at the 3' or 5' side results in a...

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Identifiers

PMID: 11937643
DOI: 10.1093/nar/30.8.e35
PMCID: PMC113230

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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