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Article

The role of spontaneous cap domain mutations in haloalkane dehalogenase specificity and evolution.

The Journal of biological chemistry - 1 Jul 1994

Pries F, van den Wijngaard A J, Bos R, Pentenga M, Janssen D B

Abstract excerpt

The first step in the utilization of the xenobiotic chlorinated hydrocarbon 1,2-dichloroethane by Xanthobacter autotrophicus is catalyzed by haloalkane dehalogenase (Dh1A). The enzyme hydrolyses 1-haloalkanes to the corresponding alcohols. This allows the organism to grow also on short-chain (C2-C4) 1-chloro-n-alkanes. We have expressed Dh1A in a strain of Pseudomonas that grows on long-chain alcohols and have...

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PMID: 8021255

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