Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Biodegradation of 1,2,3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene.

Applied and environmental microbiology - 1 Jul 2002

Bosma Tjibbe, Damborský Jirí, Stucki Gerhard, Janssen Dick B

Abstract excerpt

Using a combined strategy of random mutagenesis of haloalkane dehalogenase and genetic engineering of a chloropropanol-utilizing bacterium, we constructed an organism that is capable of growth on 1,2,3-trichloropropane (TCP). This highly toxic and recalcitrant compound is a waste product generated from the manufacture of the industrial chemical epichlorohydrin. Attempts to select and enrich bacterial cultures...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biodegradation, Environmental
Share to Biological Evolution

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Hydrolases

Share to Kinetics

Share to Mutagenesis

Share to Recombinant Proteins

Share to Rhizobium

Share to Rhodococcus

Source

Open in PubMed Open full text

Identifiers

PMID: 12089046
DOI: 10.1128/AEM.68.7.3582-3587.2002
PMCID: PMC126774

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Biodegradation of 1,2,3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene.PMID 12089046Read ↗

Mechanism of enhanced conversion of 1,2,3-trichloropropane by mutant haloalkane dehalogenase revealed by molecular modeling.Semantic proximity Read ↗

Catalytic Cycle of Haloalkane Dehalogenases Toward Unnatural Substrates Explored by Computational Modeling.Semantic proximity Read ↗

Effective use of a horizontally-transferred pathway for dichloromethane catabolism requires post-transfer refinement.Semantic proximity Read ↗

Isolation and functional analysis of a glycolipid producing Rhodococcus sp. strain IITR03 with potential for degradation of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT).Semantic proximity Read ↗

Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels.Semantic proximity Read ↗

Dichloromethane mediated in vivo selection and functional characterization of rat glutathione S-transferase theta 1-1 variants.Semantic proximity Read ↗

Genetic manipulation of the obligate chemolithoautotrophic bacterium Thiobacillus denitrificans.Semantic proximity Read ↗

Exploring the thermostable properties of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by a combinatorial directed evolution strategy.Semantic proximity Read ↗

Mutagenesis and expression of methane monooxygenase to alter regioselectivity with aromatic substrates.Semantic proximity Read ↗

A high-throughput colorimetric assay for screening halohydrin dehalogenase saturation mutagenesis libraries.Semantic proximity Read ↗

Crystal structures of native and inactivated cis-3-chloroacrylic acid dehalogenase: Implications for the catalytic and inactivation mechanisms.Semantic proximity Read ↗

Novel Rhizosphere Soil Alleles for the Enzyme 1-Aminocyclopropane-1-Carboxylate Deaminase Queried for Function with an In Vivo Competition Assay.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.