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Engineering pH-tolerant mutants of a cyanide dihydratase.

Applied microbiology and biotechnology - 1 Apr 2012

Wang Lan, Watermeyer Jean M, Mulelu Andani E, Sewell B Trevor, Benedik Michael J

Abstract excerpt

Cyanide dihydratase is an enzyme in the nitrilase family capable of transforming cyanide to formate and ammonia. This reaction has been exploited for the bioremediation of cyanide in wastewater streams, but extending the pH operating range of the enzyme would improve its utility. In this work, we describe mutants of Bacillus pumilus C1 cyanide dihydratase (CynD(pum)) with improved activity at higher pH....

Topics

Amino Acid Substitution
Bacillus
Bacterial Proteins
Enzyme Stability
Hydrogen-Ion Concentration
Hydrolases
Mutation
Protein Engineering

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 21993481
DOI: 10.1007/s00253-011-3620-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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