Article
Activation of an Asp-124-->Asn mutant of haloalkane dehalogenase by hydrolytic deamidation of asparagine.
FEBS letters - 23 Jan 1995
Pries F, Kingma J, Janssen D B
Abstract excerpt
Haloalkane dehalogenase hydrolyses various 1-halo-n-alkanes to the corresponding alcohols by covalent catalysis with formation of an alkyl-enzyme intermediate. The carboxylate function of the nucleophilic aspartate (Asp-124) that displaces the halogen during formation of the intermediate was changed to an amide by site-directed mutagenesis (Asp-124-->Asn). Activity measurements and analysis of peptides containing...
Topics
- Amides
- Amino Acid Sequence
- Asparagine
- Aspartic Acid
- Base Sequence
- DNA Primers
- Deamination
- Enzyme Activation
- Hydrolases
- Hydrolysis
- Molecular Sequence Data
- Mutation