Article
Characterization of charge change super-repressor mutants of trp repressor: effects on oligomerization conformation, ligation and stability.
Journal of molecular biology - 22 Nov 1996
Reedstrom R J, Martin K S, Vangala S, Mahoney S, Wilker E W, Royer C A
Abstract excerpt
We have carried out a physical characterization of mutant repressor proteins of the trp repressor system of Escherichia coli by circular dichroism, chemical denaturation, and 8-anilino-1-naphthalenesulfonate binding. We have also probed the protein-protein interactions via fluorescence anisotropy...
Topics
- Anilino Naphthalenesulfonates
- Bacterial Proteins
- Circular Dichroism
- DNA, Bacterial
- Escherichia coli
- Fluorescent Dyes
- Kinetics
- Ligands
- Models, Molecular
- Mutation
- Protein Binding
- Protein Conformation
- Protein Denaturation
- Protein Structure, Secondary
- Repressor Proteins
- Thermodynamics
- Tryptophan
- Urea