Article
An alkaline phosphatase protection assay to investigate trp repressor/operator interactions.
Biochemistry - 29 Jan 1991
Marmorstein R Q, Sprinzl M, Sigler P B
Abstract excerpt
We have used an alkaline phosphatase protection assay to investigate the interaction of the trp repressor with its operator sequence. The assay is based on the principle that the trp repressor will protect a terminally 5'-32P-labeled operator DNA fragment from attack by alkaline phosphatase. The optimal oligonucleotide for investigating the trp repressor/operator interaction extends two base pairs from each end...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.