Article
An alkaline phosphatase protection assay to investigate trp repressor/operator interactions.
Biochemistry - 29 Jan 1991
Marmorstein R Q, Sprinzl M, Sigler P B
Abstract excerpt
We have used an alkaline phosphatase protection assay to investigate the interaction of the trp repressor with its operator sequence. The assay is based on the principle that the trp repressor will protect a terminally 5'-32P-labeled operator DNA fragment from attack by alkaline phosphatase. The...
Topics
- Alkaline Phosphatase
- Apoproteins
- Bacterial Proteins
- Base Composition
- Base Sequence
- DNA
- Escherichia coli Proteins
- Hydrogen Bonding
- Hydrolysis
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Oligonucleotides
- Operator Regions, Genetic
- Phosphorus Radioisotopes
- Protein Conformation
- Repressor Proteins
- Tryptophan