Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Non-independence of Mnt repressor-operator interaction determined by a new quantitative multiple fluorescence relative affinity (QuMFRA) assay.

Nucleic acids research - 15 Jun 2001

Man T K, Stormo G D

Abstract excerpt

Salmonella bacteriophage repressor Mnt belongs to the ribbon-helix-helix class of transcription factors. Previous SELEX results suggested that interactions of Mnt with positions 16 and 17 of the operator DNA are not independent. Using a newly developed high-throughput quantitative multiple fluorescence relative affinity (QuMFRA) assay, we directly quantified the relative equilibrium binding constants (K(ref)) of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacteriophage P22
Share to Fluorescence
Share to Fluorescent Dyes

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Operator Regions, Genetic

Share to Salmonella

Share to Thermodynamics

Share to Viral Proteins

Share to Viral Regulatory and Accessory Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 11410653
DOI: 10.1093/nar/29.12.2471
PMCID: PMC55749

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Non-independence of Mnt repressor-operator interaction determined by a new quantitative multiple fluorescence relative affinity (QuMFRA) assay.PMID 11410653Read ↗

Specificity of Mnt 'master residue' obtained from in vivo and in vitro selections.Semantic proximity Read ↗

ProNAB: database for binding affinities of protein-nucleic acid complexes and their mutants.Semantic proximity Read ↗

The quorum sensing transcriptional regulator TraR has separate binding sites for DNA and the anti-activator.Semantic proximity Read ↗

DNA-binding specificity prediction with FoldX.Semantic proximity Read ↗

Design and optimization of SPR-based binding assay for evaluation and screening of MITF-E-box binding inhibitor.Semantic proximity Read ↗

MPRAnator: a web-based tool for the design of massively parallel reporter assay experiments.Semantic proximity Read ↗

Ligand-binding pocket bridges DNA-binding and dimerization domains of the urate-responsive MarR homologue MftR from Burkholderia thailandensis.Semantic proximity Read ↗

Escherichia coli Hfq binds A18 and DsrA domain II with similar 2:1 Hfq6/RNA stoichiometry using different surface sites.Semantic proximity Read ↗

DNA sequence-specific recognition by a transcriptional regulator requires indirect readout of A-tracts.Semantic proximity Read ↗

Structure and rational engineering of the PglX methyltransferase and specificity factor for BREX phage defenceSemantic proximity Read ↗

The biophysical characterization of the transcription factor MntR from Mycobacterium tuberculosis and its selected mutants.Semantic proximity Read ↗

Studies of the Escherichia coli Trp repressor binding to its five operators and to variant operator sequences.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.