Article
Mutant tryptophan aporepressors with altered specificities of corepressor recognition.
Genetics - 1 May 1991
Arvidson D N, Shapiro M, Youderian P
Abstract excerpt
The Escherichia coli trpR gene encodes tryptophan aporepressor, which binds the corepressor ligand, L-tryptophan, to form an active repressor complex. The side chain of residue valine 58 of Trp aporepressor sits at the bottom of the corepressor (L-tryptophan) binding pocket. Mutant trpR genes encoding changes of Val58 to the other 19 naturally occurring amino acids were made. Each of the mutant proteins requires...
Topics
- Apoproteins
- Bacterial Proteins
- Base Sequence
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation
- Genes, Bacterial
- Molecular Sequence Data
- Molecular Structure
- Mutation
- Repressor Proteins