Article
Effect of amino acid alterations in the tryptophan-binding site of the trp repressor.
The Journal of biological chemistry - 15 Jan 1990
He J J, Matthews K S
Abstract excerpt
Mutations in the tryptophan-binding site of the trp repressor have been generated using site-directed mutagenesis. The selection of sites for alteration was based on the three-dimensional x-ray crystallographic structure (Schevitz, R. W., Otwinowski, Z., Joachimiak, A., Lawson, C. L., and Sigler,...
Topics
- Amino Acid Sequence
- Amino Acids
- Circular Dichroism
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Molecular Sequence Data
- Mutation
- Repressor Proteins
- Spectrometry, Fluorescence
- Transcription Factors
- Tryptophan