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Article

A multiplasmid approach to preparing large libraries of polyketides.

Proceedings of the National Academy of Sciences of the United States of America - 12 Oct 1999

Xue Q, Ashley G, Hutchinson C R, Santi D V

Abstract excerpt

A three-plasmid system for heterologous expression of 6-deoxyerythronolide B synthase (DEBS) has been developed to facilitate combinatorial biosynthesis of polyketides made by type I modular polyketide synthases (PKSs). The eryA PKS genes encoding the three DEBS subunits were individually cloned into three compatible Streptomyces vectors carrying mutually selectable antibiotic resistance markers. A strain of...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 10518520
DOI: 10.1073/pnas.96.21.11740
PMCID: PMC18356

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