Article
Engineered biosynthesis of a complete macrolactone in a heterologous host.
Science (New York, N.Y.) - 22 Jul 1994
Kao C M, Katz L, Khosla C
Abstract excerpt
Macrocyclic polyketides have been subjects of great interest in synthetic and biosynthetic chemistry because of their structural complexity and medicinal activities. With expression of the entire 6-deoxyerythronolide B synthase (DEBS) (10,283 amino acids) in a heterologous host, substantial quantities of 6-deoxyerythronolide B (6dEB), the aglycone of the macrolide antibiotic erythromycin, and...
Topics
- Acyl Coenzyme A
- Base Sequence
- Binding Sites
- Cloning, Molecular
- Drug Design
- Erythromycin
- Escherichia coli
- Genes, Bacterial
- Genetic Engineering
- Genetic Vectors
- Molecular Sequence Data
- Multienzyme Complexes