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Article

Allele-specific polymerase chain reaction for genotyping human cytochrome P450 2E1.

Journal of clinical laboratory analysis - 1 Jan 1999

Sohda T

Abstract excerpt

Allele-specific polymerase chain reaction (AS-PCR) was applied to investigate the cytochrome P450 2E1 (CYP2E1) genotype. AS-PCR is a competitive multiplex PCR method in which PCR amplification is successfully performed only by using the sequence of 3' oligonucleotide ends as a DNA template in order to obtain an absolutely complementary product. I was able to produce allele-specific primers whose 3' ends had the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Polymorphism, Restriction Fragment Length

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Identifiers

PMID: 10494127
PMCID: PMC6807827

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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