Article
Apolipoprotein E genotyping using the polymerase chain reaction and allele-specific oligonucleotide primers.
Journal of lipid research - 1 Jan 1991
Main B F, Jones P J, MacGillivray R T, Banfield D K
Abstract excerpt
A method for apolipoprotein (apo) E genotyping was developed using the polymerase chain reaction (PCR) with allele-specific oligonucleotide primers (ASP). Synthetic oligonucleotides with base-pair mismatches at the 3' terminus were used as primers to amplify the apoE gene in subjects previously phenotyped using isoelectric focusing (IEF). Complementary primer-allele combinations were specifically amplified by...
Topics
- Alleles
- Amino Acid Sequence
- Apolipoproteins E
- Base Sequence
- DNA
- Genetic Techniques
- Genotype
- Humans
- Molecular Sequence Data
- Oligonucleotides
- Polymerase Chain Reaction