Article
Duplex polymerase chain reaction with confronting two-pair primers (PCR-CTPP) for genotyping alcohol dehydrogenase beta subunit (ADH2) and aldehyde dehydrogenase 2 (ALDH2).
Alcohol and alcoholism (Oxford, Oxfordshire) - 1 Jan 2000
Tamakoshi Akiko, Hamajima Nobuyuki, Kawase Haruya, Wakai Kenji, Katsuda Nobuyuki, Saito Toshiko, Ito Hidemi, Hirose Kaoru, Takezaki Toshiro, Tajima Kazuo
Abstract excerpt
AIMS: Alcohol dehydrogenase beta subunit (ADH2) Arg47His and aldehyde dehydrogenase 2 (ALDH2) Glu487Lys were genotyped by a duplex polymerase chain reaction (PCR) with confronting two-pair primers (PCR-CTPP), which allows DNA amplification with one-tube PCR including eight primers, and subsequent electrophoresis. METHODS: Several PCR conditions were tested to establish the optimal conditions for distinguishing...
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