Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Recovery of upstream cDNA sequences by a PCR-based biotin-capture method.

BioTechniques - 1 Sept 1999

Gräslund S, Larsson M, Sterky F, Uhlén M, Lundeberg J, Höög C, Ståhl S

Abstract excerpt

The world-wide, large-scale sequencing efforts have generated an abundance of partial cDNA sequences, i.e., expressed sequence tags (ESTs), accessible in the public databases. To enable functional characterization of these partial cDNA sequences, general and robust methods for recovery of upstream full-coding cDNA sequences are needed. Here, a novel biotin- and PCR-assisted capture method was used directly on...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biotin
Share to Electrophoresis, Agar Gel
Share to Magnetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Microspheres

Share to RNA-Directed DNA Polymerase

Share to RNA Splicing

Share to Streptavidin

Share to Testis

Source

Identifiers

PMID: 10489608

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Recovery of upstream cDNA sequences by a PCR-based biotin-capture method.PMID 10489608Read ↗

Identification of differentially expressed 5'-end mRNA variants by an improved RACE technique (PEETA).Semantic proximity Read ↗

Two cat expression vectors for cloning and generation of 3'- and 5'-deletion mutants.Semantic proximity Read ↗

PCR-assisted cDNA cloning: a guided tour of the minefield.Semantic proximity Read ↗

Target preparation for genotyping specific genes or gene segments.Semantic proximity Read ↗

Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.Semantic proximity Read ↗

Protein truncation test.Semantic proximity Read ↗

Cell-free synthesis of recombinant proteins from PCR-amplified genes at a comparable productivity to that of plasmid-based reactions.Semantic proximity Read ↗

DSN depletion is a simple method to remove selected transcripts from cDNA populations.Semantic proximity Read ↗

PCR amplification of mRNA directly from a crude cell lysate prepared by thermophilic protease digestion.Semantic proximity Read ↗

A method to identify cDNAs based on localization of green fluorescent protein fusion products.Semantic proximity Read ↗

Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.Semantic proximity Read ↗

Development of EST-SSRs in Japanese scallop (Mizuhopecten yessoensis) from cDNA libraries.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.