Article
Target preparation for genotyping specific genes or gene segments.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Petersen Jesper, Poulsen Lena, Dufva Martin
Abstract excerpt
Generation of single stranded target is of high importance for hybridization reactions on oligonucleotide microarrays. Several methods have been established for production of single stranded DNA and in vitro transcribed RNA. Here we describe three robust methods for target amplification from purified genomic DNA or pre-amplified DNA. The protocols include incorporation of biotin labels in the target molecules and...
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