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Article

DSN depletion is a simple method to remove selected transcripts from cDNA populations.

Molecular biotechnology - 1 Mar 2009

Bogdanova Ekaterina A, Shagina Irina A, Mudrik Elena, Ivanov Igor, Amon Peter, Vagner Laura L, Lukyanov Sergey A, Shagin Dmitry A

Abstract excerpt

A novel DSN-depletion method allows elimination of selected sequences from full-length-enriched cDNA libraries. Depleted cDNA can be applied for subsequent EST sequencing, expression cloning, and functional screening approaches. The method employs specific features of the kamchatka crab duplex-specific nuclease (DSN). This thermostable enzyme is specific for double-stranded (ds) DNA, and is thus used for...

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PMID: 19127453
DOI: 10.1007/s12033-008-9131-y

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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