Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.

doi:10.1073/pnas.88.5.1731

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Article

Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.

Proceedings of the National Academy of Sciences of the United States of America - 1 Mar 1991

Elledge S J, Mulligan J T, Ramer S W, Spottswood M, Davis R W

Abstract excerpt

This work describes a multifunctional phage lambda expression vector system, lambda YES, designed to facilitate gene isolation from eukaryotes by complementation of Escherichia coli and Saccharomyces cerevisiae mutations. lambda YES vectors have a selection for cDNA inserts using an oligo adaptor...

Topics

Antigens, Differentiation, T-Lymphocyte
Bacteriophage lambda
Base Sequence
CD28 Antigens
Cloning, Molecular
DNA, Viral
Escherichia coli
Gene Library
Genes
Genetic Complementation Test
Genetic Vectors

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Molecular Sequence Data

Mutation

Oligonucleotide Probes

Plants

Plasmids

Restriction Mapping

Source

Open in PubMed Open full text

Identifiers

PMID: 1848010
DOI: 10.1073/pnas.88.5.1731
PMCID: PMC51098

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1848010-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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