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Article

A method to identify cDNAs based on localization of green fluorescent protein fusion products.

Proceedings of the National Academy of Sciences of the United States of America - 28 Mar 2000

Misawa K, Nosaka T, Morita S, Kaneko A, Nakahata T, Asano S, Kitamura T

Abstract excerpt

We previously established a high-efficiency, retrovirus-mediated expression cloning method. Using this system, we now have developed an expression cloning method (FL-REX; fluorescence localization-based retrovirus-mediated expression cloning) in which cDNAs can be isolated based on the subcellular localization of their protein products. Complementary DNAs generated from mRNA using random hexamers were fused to...

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Identifiers

PMID: 10716735
DOI: 10.1073/pnas.97.7.3062
PMCID: PMC16192

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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