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Article

Identification of differentially expressed 5'-end mRNA variants by an improved RACE technique (PEETA).

Nucleic acids research - 1 Aug 1999

Flouriot G, Brand H, Gannon F

Abstract excerpt

A rapid and efficient procedure is described for mapping and cloning the 5'-ends of mRNAs, including those generated from a unique gene by alternative splicing and promoter usage. This method involves reverse transcription of the targeted mRNAs from a long, highly labeled specific primer, resolution of the extension products on a DNA sequencing gel, elution and poly(dC) tailing of the single-stranded cDNAs of...

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Share to Estrogen Receptor alpha
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Cloning, Molecular

Share to DNA, Complementary

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Identifiers

PMID: 10454627
DOI: 10.1093/nar/27.15.e8
PMCID: PMC148519

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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