Article
Protein truncation test.
Current protocols in human genetics - 1 Sept 2004
Vossen Rolf, den Dunnen Johan T
Abstract excerpt
The protein truncation test detects mutations at the protein level that lead to premature translation termination. The method has evolved considerably since is original publication in this manual. This thoroughly revised unit describes what is now the preferred method for performing the protein truncation test. Transcription and translation are performed in separate reactions; during translation, biotin-labeled...
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