Article
Knockout of one acetylcholinesterase allele in the mouse.
Chemico-biological interactions - 14 May 1999
Xie W, Wilder P J, Stribley J, Chatonnet A, Rizzino A, Taylor P, Hinrichs S H, Lockridge O
Abstract excerpt
One allele of the AChE gene (ACHE) was knocked out in embryonic stem (ES) cells by homologous recombination. The targeting vector contained 2 kb of a TK gene cassette for negative selection, 884 bp of ACHE including exon 1, 1.6 kb of a Neo(r) gene cassette for positive selection, 5.2 kb of the ACHE Bam HI fragment including exon 6, and 3 kb of Bluescript. The use of this vector deleted exons 2-5, which removed...
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