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Article

Agouti C57BL/6N embryonic stem cells for mouse genetic resources.

Nature methods - 1 Jul 2009

Pettitt Stephen J, Liang Qi, Rairdan Xin Y, Moran Jennifer L, Prosser Haydn M, Beier David R, Lloyd Kent C, Bradley Allan, Skarnes William C

Abstract excerpt

We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic...

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Identifiers

PMID: 19525957
DOI: 10.1038/nmeth.1342
PMCID: PMC3555078

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