Article
A tool for functional plant genomics: chimeric RNA/DNA oligonucleotides cause in vivo gene-specific mutations.
Proceedings of the National Academy of Sciences of the United States of America - 20 Jul 1999
Beetham P R, Kipp P B, Sawycky X L, Arntzen C J, May G D
Abstract excerpt
Self-complementary chimeric oligonucleotides (COs) composed of DNA and modified RNA residues were evaluated as a means to (i) create stable, site-specific base substitutions in a nuclear gene and (ii) introduce a frameshift in a nuclear transgene in plant cells. To demonstrate the creation of allele-specific mutations in a member of a gene family, COs were designed to target the codon for Pro-196 of SuRA, a...
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