Article
Spontaneous mutation frequency in plants obscures the effect of chimeraplasty.
Plant molecular biology - 1 Nov 2003
Ruiter René, van den Brande Ilse, Stals Ellen, Delauré Stijn, Cornelissen Marc, D'Halluin Kathleen
Abstract excerpt
In this study we tested the performance of chimeraplasts, chimeric RNA/DNA oligonucleotides, for the creation of directed changes in chromosomal sequences in tobacco and oilseed rape. As target genes for chimeraplasty, the endogenous als gene and two transgenes, bar and a fusion between egfp and bar, were used. In experiments in which similar numbers of cells were treated with and without chimeraplasts, delivery...
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