Article
A selection strategy in plant transformation based on antisense oligodeoxynucleotide inhibition.
The Plant journal : for cell and molecular biology - 1 Mar 2014
Xie Zhoupeng, Sundström Jens F, Jin Yunkai, Liu Chunlin, Jansson Christer, Sun Chuanxin
Abstract excerpt
Antisense oligodeoxynucleotide (asODN) inhibition was developed in the 1970s, and since then has been widely used in animal research. However, in plant biology, the method has had limited application because plant cell walls significantly block efficient uptake of asODN to plant cells. Recently, we have found that asODN uptake is enhanced in a sugar solution. The method has promise for many applications, such as...
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