Article
In Vivo Chemical Genomics with Random Cyclized Peptides.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021
Shuipys Tautvydas, Clancy Maureen, Estrada-Johnson Elizabeth, Folta Kevin M
Abstract excerpt
We have developed and applied a novel strategy that can best be described as in vivo chemical genomics, a concept where populations of any transformable organism may be screened for consequences of novel RNAs or peptides. We created a library of ~800,000 random DNA sequences biased only by third-position nucleotide substitutions that suppress the frequency of termination codons. The sequences may be shuttled to...
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